id: 36839
Title: Production of functional antibodies from hyperimmune duck eggs for neutralization of new coronavirus SARS CoV-2.
Authors: Melnychuk M.D., Sereda O.V., Yu W., Spyrydonov V.G.
Keywords: COVID-19, SARS CoV-2, yolk immunoglobulin, virus neutralizing antibodies, blocking ELISA, coronavirus vaccine
Date of publication: 2024-09-05 13:35:42
Last changes: 2024-09-05 13:35:42
Year of publication: 2024
Summary: To develop a technology for producing coronavirus-neutralizing antibodies in duck eggs (Anas platyrhynchos) immunized with a recombinant fragment of the S-antigen of the SARS CoV-2 virus, and to analyze the possibility of their use as an active substance in the develop- ment of local inhalation drugs to prevent the aerosol spread of respiratory viral infections, including a new coronavirus. Methods. Recombinant antigen production in the yeast expres- sion system, production of antibodies from duck eggs, affinity purification, ELISA. Results. We describe an efficient method for obtaining functional yolk antibodies (IgY) from the hy- perimmune duck eggs. We have shown that the average yield of total yolk antibodies from duck eggs is 0.25±0.05 grams per yolk. The obtained antibodies showed the virus neutraliza- tion activity at 70.6 % inhibition (PI) in blocking ELISA, which was comparable to the human serum antibodies after vaccination with coronavirus vaccine PI at 87 % (p≤0.05). Conclusions. The results obtained indicate that S-specific yolk antibodies can be used as an active substance for the development of topical inhalation drugs that prevent coronavirus infection.
URI: http://repository.vsau.org/repository/getfile.php/36839.pdf
Publication type: Статті Scopus
Publication: Biopolymers and Cell. 2024. Vol. 40, Issue 2. P. 109-117. DOI: http://dx.doi.org/10.7124/bc.000AB1
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